Recombinant Human MMP-9/CLG4B  (CI71)

产品说明(Description)

Recombinant Human Matrix metalloproteinase-9 is produced by our Mammalian expression system and the target gene encoding Ala19-Asp707 is expressed with a 6His tag at the C-terminus. 

Accession #: AAH06093.1

Known as: Matrix metalloproteinase-9;92 kDa gelatinase;92 kDa type IV collagenase;Gelatinase B;MMP9

制剂（Formulation）

Supplied as a 0.2 μm filtered solution of 20mM Tris-Hcl, 2mM CaCl2, 150mM NaCl, 0.05% Brij35(w/v), pH 7.5.

Note: The proenzyme needs to be activated by APMA.

质量控制（Quality Control） 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

运输(Shipping)

The product is shipped on dry ice/polar packs. 
Upon receipt, store it immediately at the temperature listed below.

保存(Storage)

Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening. 
Please minimize freeze-thaw cycles.     

背景（Background）

Matrix metallopeptidase 9 (MMP-9) is an enzyme encoded by the MMP9 gene. This protein, which is produced by normal alveolar macrophages and granulocytes, can be activated by 4-aminophenylmercuric acetate and phorbol ester and up-regulated by ARHGEF4, SPATA13 and APC via the JNK signaling pathway in colorectal tumor cells. MMP-9 is involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, angiogenesis, bone development, wound healing, cell migration, learning and memory, as well as in pathological processes, such as arthritis, intracerebral hemorrhage, and metastasis.

电泳(SDS-PAGE)

参考文献（References）

Cloning and characteristic of MMP1 gene from Hyriopsis cumingii and collagen hydrolytic activity of its recombinant protein[J]. BaoqingHu. et al. Gene. 2019.

FOR RESEARCH USE ONLY