DMEM/F12

产品概述
DMEM/F12培养基是在DMEM培养基的基础上,添加F12培养基中更为丰富的营养成分,含有多种微量元素,广泛应用于多种哺乳类细胞的培养。同时,DMEM/F12培养基常作为开发无血清培养基的基础,也适用于低血清含量下哺乳动物细胞的培养以及克隆密度培养。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分,但不含蛋白质、脂类或任何生长因子,故此产品需搭配血清或无血清添加物使用。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
PH | 7.2~7.4 |
L-谷氨酰胺 | 2.5mM |
NaHCO3 | 2438mg/L |
D-葡萄糖 | 3151mg/L |
丙酮酸钠 | 0.5mM |
HEPES缓冲剂 | 无 |
酚红指示剂 | 8.1 mg/L |
储存条件 | 2~8℃,避光 |
运输条件 | 常温 |
有效期 | 12个月 |
注意事项
1、本产品经过滤除菌,使用时应注意无菌操作,避免污染;
2、为保持本产品的最佳使用效果,请勿进行冻融处理;
3、本产品仅用于科研或进一步研究使用,不用于诊断和治疗。
参考文献
Fabrication, bacteriostasis and osteointegration properties researches of the additively-manufactured porous tantalum scaffolds loading vancomycin(2023/01/03)
作者:Hu Qian, Ting Lei, Long Hua
期刊:Bioactive Materials
DOI:10.1016/j.bioactmat.2022.12.013
影响因子 :16.8740
引用产品:PM150312
Architecture-engineered electrospinning cascade regulates spinal microenvironment to promote nerve regeneration(2023/01/18)
作者:Yu Tang, Zonghan Xu, Jincheng Tang
期刊:Advanced Healthcare Materials
影响因子 :11.0920
MUC1 promotes glioblastoma progression and TMZ resistance by stabilizing EGFRvIII(2022/12/11)
作者:Fei Tong, Jixing Zhao, Ziyuan Fang
期刊:PHARMACOLOGICAL RESEARCH
DOI:10.1016/j.phrs.2022.106606
影响因子 :10.3340
A novel taxane, difluorovinyl-ortataxel, effectively overcomes paclitaxel-resistance in breast cancer cells(2020/07/27)
作者:Dade Rong, Changwei Wang, Xiaomei Zhang
期刊:Cancer Letters
DOI:10.1016/j.canlet.2020.06.025
影响因子 :9.756
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作者:Zhang, Yuan, Ding, Yuhao, Zhao, Huizi, Wang, Zhonghao, Zeng, Fanle, Qian, Zhenzhen, Li, Jun, Ma, Taotao, Huang, Cheng
期刊:Cell Death & Disease
DOI:10.1038/s41419-022-05422-3
影响因子 :9.6850
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Decitabine enhances targeting of AML cells by NY-ESO-1-specific TCR-T cells and promotes the maintenance of effector function and the memory phenotype(2022/09/12)
作者:Synat Kang, Lixin Wang, Lu Xu
期刊:ONCOGENE
DOI:10.1038/s41388-022-02455-y
影响因子 :8.7560
引用产品:PM150312
Multi-omics characterization of a scoring system to quantify hypoxia patterns in patients with head and neck squamous cell carcinoma(2023/01/10)
作者:Cong Peng, Huiping Ye, Zhengyang li
期刊:Journal of Translational Medicine
DOI:10.1186/s12967-022-03869-8
影响因子 :8.4400
Exosomes derived from human umbilical cord mesenchymal stem cells ameliorate experimental non-alcoholic steatohepatitis via Nrf2/NQO-1 pathway(2022/09/10)
作者:Yaxing Kang, Yiran Song, Yuxin Luo
期刊:FREE RADICAL BIOLOGY AND MEDICINE
DOI:10.1016/j.freeradbiomed.2022.08.037
影响因子 :8.1010
A Step-by-Step Refined Strategy for Highly Efficient Generation of Neural Progenitors and Motor Neurons from Human Pluripotent Stem Cells(2021/11/9)
作者:Jie Ren, Chaoyi Li, Mengfei Zhang
期刊:Cells
影响因子 :7.6660
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作者:Hailin Zhou, Zhongyuan Qi, Pei Pei
期刊:Biomaterials Science
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作者:Xianliang Luo, Wangxin Liu, Minjie Zhao, Tao Liu, Feifei Xiong, Lei Lei, Fuhuai Jia, Fengqin Feng
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DOI:10.1016/j.foodres.2022.112148
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作者:Kai Xue,Yurong Wang,Yan Wang
期刊:Oxidative Medicine and Cellular Longevity
影响因子 :7.3100
Upregulation of long intergenic non-coding RNA LINC00326 inhibits non-small cell lung carcinoma progression by blocking Wnt/β-catenin pathway through modulating the miR-657/dickkopf WNT signaling pathway inhibitor 2 axis(2023/02/06)
作者:Yingqian Zhang, Jiao Yuan, Mengfei Guo
期刊:Biology Direct
DOI:10.1186/s13062-023-00359-9
影响因子 :7.1730
Ciprofloxacin and enrofloxacin can cause reproductive toxicity via endocrine signaling pathways(2022/09/02)
作者:Lirui Hou, Yuhan Fu, Chong Zhao
期刊:ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI:10.1016/j.ecoenv.2022.114049
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作者:Limin Zhang, Qingxin Gao, Jun Chen
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作者:Yan Li, Nianling Yao, Yan Gao
期刊:Bioengineered
DOI:10.1080/21655979.2021.1987125
影响因子 :6.8320
MicroRNA miR-106a-5p targets forkhead box transcription factor FOXC1 to suppress the cell proliferation, migration, and invasion of ectopic endometrial stromal cells via the PI3K/Akt/mTOR signaling pathway(2021/6/4)
作者:Xinyue Zhou, Zhenyu Chen, Lipeng Pei
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DOI:10.1080/21655979.2021.1933679
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作者:Yu Zhang, Mingcun Li, Weixing Wang
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DOI:10.1080/21655979.2021.2012627
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DOI:10.1080/21655979.2022.2054207
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Exosomal lncRNA NEAT1 from cancer-associated fibroblasts facilitates endometrial cancer progression via miR-26a/b-5p-mediated STAT3/YKL-40 signaling pathway(2021/06/18)
作者:Jiangtao Fan, Zhaoyu Zhou, Yanlu Luo
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作者:Zhenning Zhang, Hai Liu, Mimi Liu
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作者:Yang Lu, Cheng Chen, Heng Wang
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DOI:10.1016/j.intimp.2022.109322
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作者:Jinjin Li, Jie Li, Hongbo Fang
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DOI:10.1016/j.intimp.2022.109185
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作者:Bin Liu, Jinchao Wang, Guangchuan Wang
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DOI:10.1016/j.intimp.2022.109605
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Veratric acid alleviates liver ischemia/reperfusion injury by activating the Nrf2 signaling pathway(2021/11/05)
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DOI:10.1016/j.intimp.2021.108294
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