TM3 (小鼠睾丸间质细胞) (种属鉴定正确)

产品信息
名称 | TM3 (小鼠睾丸间质细胞) (种属鉴定正确) |
别称 | TM-3 |
种属 | 小鼠 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
生长培养基 | DMEM/F12+5% HS+2.5% FBS+1% P/S |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO温度:液氮 |
培养条件 | 气相:空气,95%;CO2,5%温度:37℃ |
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2~3次/周 |
注意事项 | 该细胞贴壁松散,操作时请尽量轻柔;换液时需预热培养基;收货如有大块脱落的细胞团,为正常现象,请按照收货注意事项处理。 |
背景描述 | TM3细胞在LH作用下cAMP产量升高,但对促卵泡激素没有响应。TM3细胞对LH的响应持续时间与血清批次有关;在LH存在下,TM3细胞可以代谢胆固醇。 |
年龄(性别) | 雄性,11-13天 |
组织来源 | 睾丸间质 |
细胞类型 | 自发永生化细胞 |
生物安全等级 | 1 |
致瘤性 | No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
受体表达情况 | Luteinizing hormone (LH); epidermal growth factor (EGF); androgen receptor, positive; estrogen receptor, positive; progesterone receptor, positive |
基因表达情况 | prostaglandin F2a |
保藏机构 | ATCC; CRL-1714 ECACC; 91060526 |
STR鉴定
参考文献
Prenatal exposure to environmentally relevant levels of PBDE-99 leads to testicular dysgenesis with steroidogenesis disorders(2021/10/20)
作者:Tianxin Zhao, Xiangliang Tang, Dian Li
期刊:Journal of Hazardous Materials
DOI:10.1016/j.jhazmat.2021.127547
影响因子 :14.2240
引用产品:CL-0234
Oxidized-LDL inhibits testosterone biosynthesis by affecting mitochondrial function and the p38 MAPK/COX-2 signaling pathway in Leydig cells(2020/08/14)
作者:Jun Jing, Ning Ding, Dandan Wang
期刊:Cell Death & Disease
DOI:10.1038/s41419-020-02751-z
影响因子 :9.685
引用产品:CL-0234
A Protein from Dioscorea polystachya (Chinese Yam) Improves Hydrocortisone-Induced Testicular Dysfunction by Alleviating Leydig Cell Injury via Upregulation of the Nrf2 Pathway(2021/11/30)
作者:Shiting Yu,Bing Han,Xin Xing
期刊:Oxidative Medicine and Cellular Longevity
影响因子 :7.3100
1,25-Dihydroxyvitamin D deficiency accelerates male reproductive senescence in aging mice and 1,25(OH)2D3 alleviates oxidative stress via NF-κB/SOD(2021/4/7)
作者:Jialing He, Hui Wang, Jiaxin Shi
期刊:American Journal Of Physiology-Endocrinology And Metabolism
DOI:10.1152/ajpendo.00531.2020
影响因子 :5.9000
引用产品:CL-0234
Melatonin inhibits apoptosis in mouse Leydig cells via the retinoic acid-related orphan nuclear receptor α/p53 pathway(2020/2/13)
作者:Zhiqiang Li, Jing Zhao, Hongyu Liu
期刊:Life Sciences
影响因子 :5.0370
引用产品:CL-0234
Melatonin Inhibits Apoptosis and Oxidative Stress of Mouse Leydig Cells via a SIRT1-Dependent Mechanism(2019/8/25)
作者:Gaoqing Xu, Jing Zhao, Hongyu Liu
期刊:Molecules.
DOI:10.3390/molecules24173084.
影响因子 :4.9270
引用产品:CL-0234
BMAL1 knockdown promoted apoptosis and reduced testosterone secretion in TM3 Leydig cell line(2020/4/16)
作者:He Ding, Jing Zhao, Hongyu Liu
期刊:Gene
DOI:10.1016/j.gene.2020.144672
影响因子 :2.9840
引用产品:CL-0234
Melatonin inhibits autophagy in TM3 cells via AKT/FOXO1 pathway(2022/01/08)
作者:Zhiqiang Li, Hongtao Wang, Kaiyan Zhang
期刊:Molecular Biology Reports
DOI:10.1007/s11033-021-07107-0
影响因子 :2.7420
引用产品:CL-0234
MicroRNA profiling reveals the role of miR-133b-3p in promoting apoptosis and inhibiting cell proliferation and testosterone synthesis in mouse TM3 cells(2023/01/30)
作者:Chengshuang Pan, Qianjin Fei, Jianyuan Jin
期刊:IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
DOI:10.1007/s11626-022-00745-z
影响因子 :2.7230
引用产品:CL-0234