Recombinant Mouse GM-CSF
Recombinant Mouse GM-CSF
产品描述
Recombinant Mouse Granulocyte-Macrophage Colony-Stimulating Factor is produced by our E.coli expression system and the target gene encoding Ala18-Lys141 is expressed.
蛋白编号P01587
产品别称Granulocyte-macrophage colony-stimulating factor; Csf2; GM-CSF; Colony-stimulating factor; Csfgm
分子量14.2 KDa
表观分子量15 KDa, reducing conditions
剂型描述Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 1mM EDTA, pH 8.0.
内毒素Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
纯度-SDS-PAGE
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)纯度-SEC-HPLC
Greater than 95% as determined by SEC-HPLC. (Regularly tested)生物活性-Cell Based Assay
Measured in a cell proliferation assay using FDC-P1 cells.The ED50 for this effect is 15-50 pg/ml. (QC verified)复溶- Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
- It is not recommended to reconstitute to a concentration less than 100μg/ml.
- Dissolve the lyophilized protein in distilled water.
- Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存条件- Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
- Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
- Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
运输条件- The product is shipped at ambient temperature.
- Upon receipt, store it immediately at the temperature listed below.
产品背景Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factorthat can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by anumber of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cellsand fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophageprogenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. Onmature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has a functional role on nonhematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF canalso stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma andadenocarcinoma cell lines.
