Calcein (Synonyms: 钙黄绿素; Fluorexon)
Calcein (Synonyms: 钙黄绿素; Fluorexon)
| 生物活性 | Calcein is a fluorescent dye and self-quenching probe, used as an indicator of lipid vesicle leakage, and also as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium. | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 体外研究 (In Vitro) | Calcein accumulates intracellularly in coelomocytes incubated in coelomic fluid or ISO-EDTA. Coelomocytes incubated in CF show an increase in the calcein fluorescence intensity compared to the control. MCE has not independently confirmed the accuracy of these methods. They are for reference only. | ||||||||||||
| 分子量 | 622.53 | ||||||||||||
| 性状 | 固体 | ||||||||||||
| Formula | C30H26N2O13 | ||||||||||||
| CAS 号 | |||||||||||||
| Emission (Em) | 515 | ||||||||||||
| Excitation (Ex) | 489 | ||||||||||||
| 中文名称 | 钙黄绿素 | ||||||||||||
| 运输条件 | Room temperature in continental US; may vary elsewhere. | ||||||||||||
| 储存方式 | 4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) | ||||||||||||
| 溶解性数据 | In Vitro: 0.1 M NaOH 中的溶解度 : 10 mg/mL (16.06 mM; ultrasonic and adjust pH to 11 with NaOH) DMSO 中的溶解度 : 5 mg/mL (8.03 mM; 超声助溶 (<60°C); 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO) 配制储备液
查看完整储备液配制表 * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) In Vivo: 请根据您的 实验动物和给药方式 选择适当的溶解方案。 以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂： 该产品建议参考如下动物实验溶解方案，可直接配制工作。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。
|
