RUNX2 Polyclonal antibody
经过测试的应用
| Positive WB detected in | ROS1728 cells, MCF-7 cells |
| Positive IF detected in | mouse brain tissue |
推荐稀释比
| APPLICATION | DILUTION |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
产品信息
20700-1-AP targets RUNX2 in WB, IHC, IF, chIP, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Applications | IF, WB, ELISA |
| Cited Applications | ChIP, IF, IHC, WB |
| Tested Reactivity | Human, Rat, Mouse |
| Cited Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | runt-related transcription factor 2 |
| Synonyms | AML3, CBF alpha 1, CBFA1, CCD, CCD1, Oncogene AML 3, OSF 2, OSF2, PEA2 alpha A, PEA2aA, PEBP2 alpha A, PEBP2A, PEBP2A1, PEBP2A2, PEBP2aA, PEBP2aA1, RUNX2 |
| Calculated molecular weight | 57 kDa |
| Observed molecular weight | 57-60 kDa |
| GenBank accession number | NM_004348 |
| Gene symbol | RUNX2 |
| Uniprot | Q13950 |
| Gene ID (NCBI) | 860 |
| RRID | AB_2722783 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RUNX2, also named as AML3, CBFA1, OSF2 and PEBP2A, is a Transcription factor which involved in osteoblastic differentiation and skeletal morphogenesis. It is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. RUNX2 inhibits MYST4-dependent transcriptional activation. The antibody is specific to RUNX2.
实验方案
| PRODUCT SPECIFIC PROTOCOLS | |
|---|---|
| WB protocol for RUNX2 antibody 20700-1-AP | Download protocol |
| IF protocol for RUNX2 antibody 20700-1-AP | Download protocol |
| STANDARD PROTOCOLS | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| SPECIES | APPLICATION | TITLE |
|---|---|---|
Arterioscler Thromb Vasc Biol Potential Role of H-Ferritin in Mitigating Valvular Mineralization. | ||
J Mech Behav Biomed Mater Citric acid enhances the physical properties, cytocompatibility and osteogenesis of magnesium calcium phosphate cement. | ||
J Cell Physiol Enhancement of tenogenic differentiation of rat tendon-derived stem cells by biglycan. | ||
BMC Oral Health ZnO nanoparticles inhibit the activity of Porphyromonas gingivalis and Actinomyces naeslundii and promote the mineralization of the cementum. | ||
J Periodontal Res Impact of Resolvin D1 on the inflammatory phenotype of periodontal ligament cell response to hypoxia | ||
Mater Today Bio Sustained release of naringin from silk-fibroin-nanohydroxyapatite scaffold for the enhancement of bone regeneration. |
