品名：标准巨噬细胞消耗试剂盒（Clodrosome®+Encapsome®） Standard Macrophage Depletion Kit (Clodrosome® + Encapsome®) 

货号：CLD-8901 

规格：2ml,5ml,10ml,15ml,20ml,25ml

 Clodrosome®是一种多层脂质体悬浮液，其中氯膦酸盐被封装在脂质体的水性隔室中。Encapsome®的配方和制备与Clodrosome®相同，只是脂质体中未添加氯膦酸盐。脂质体通过2μm聚碳酸酯膜过滤，以确保从悬浮液中去除可能对动物有毒的较大颗粒。两者都是在无菌条件下制备和包装的。当动物或细胞用Clodrosome®处理时，吞噬细胞将脂质体识别为入侵的外来颗粒，并通过吞噬作用将脂质体从局部组织或血清中去除。脂质体随后将氯膦酸盐释放到胞质溶胶中，导致细胞死亡。未包封的氯膦酸盐不能穿过细胞膜引发细胞死亡。 对照脂质体（Encapsome®）通过与Clodrosome®相同的机制被识别和吞噬。由于对照脂质体不含氯膦酸盐，吞噬细胞不会被杀死。然而，吞噬细胞确实通过细胞因子分泌、吞噬活性的暂时暂停和文献中描述的其他反应对对照脂质体的摄入做出反应。 巨噬细胞耗竭试剂盒由一小瓶Clodrosome®和一小瓶Encapsome®组成。例如，5毫升试剂盒由5毫升Clodrosome®和5毫升Encapsome®组成。 m-Clodrosome®和m-Encapsome®是甘露糖基试剂，专门配制用于有效靶向中枢神经系统中的巨噬细胞和含有更多甘露糖受体的巨噬细胞。有关这些试剂的更多信息，请参阅此处。 适用于巨噬细胞靶向和追踪的荧光脂质体（氟脂质体®）含有五种不同的荧光染料（DiI、DiO、DiD、DiA和DiR），覆盖整个光谱。荧光脂质体有标准形式和甘露糖基形式。

 Clodrosome® is a multilamellar liposome suspension in which clodronate is encapsulated in the aqueous compartments of the liposomes. Encapsome® is formulated and prepared identically to Clodrosome® except that clodronate is not added to the liposomes. The liposomes are filtered through 2 μm polycarbonate membranes to ensure that the larger particles, which may be toxic to animals, are removed from the suspension. Both are prepared and packaged under sterile conditions. When animals or cells are treated with Clodrosome®, phagocytic cells recognize the liposomes as invading foreign particles and proceed to remove the liposomes from the local tissue or serum via phagocytosis. The liposomes then release clodronate into the cytosol, resulting in cell death. Non-encapsulated clodronate cannot cross the cell membrane to initiate cell death. Control liposomes (Encapsome®) are recognized and phagocytosed by the same mechanism as Clodrosome®. Since the control liposomes do not contain clodronate, the phagocytic cells are not killed. However, phagocytes do respond to the ingestion of control liposomes by cytokine secretion, temporary suspension of phagocytic activity and other responses described in the literature. Macrophage depletion kit is composed of one vial of Clodrosome® and one vial of Encapsome®. As an example, a 5-ml kit is composed of 5 ml Clodrosome® and 5 ml Encapsome®. m-Clodrosome® and m-Encapsome® are mannosylated reagents that are specifically formulated to efficiently target the macrophages in central nervous systems and macrophages that contain more mannose receptors. For more information about these reagent see here. Fluorescent liposomes (Fluoroliposome®) suitable for macrophage targeting and tracking are available containing five different fluorescent dyes (DiI, DiO, DiD, DiA and DiR) that covers the entire spectrum. Fluorescent liposomes come in standard and mannosylated form.