Osteocalcin Polyclonal antibody
经过测试的应用
| Positive IHC detected in | human osteosarcoma tissue, human testis tissue, mouse kidney tissue, mouse testis tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | NIH/3T3 cells |
推荐稀释比
| APPLICATION | DILUTION |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
产品信息
The immunogen of 23418-1-AP is Osteocalcin Fusion Protein expressed in E. coli.
| Tested Applications | IF, IHC, ELISA |
| Cited Applications | IF, IHC |
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | Human, Mouse, Rat, Pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Osteocalcin fusion protein Ag20065 |
| Full Name | bone gamma-carboxyglutamate (gla) protein |
| Synonyms | BGLAP, Osteocalcin, BGP, Bone Gla protein, OC, OCN, Osteocalcin, PMF1 |
| Calculated molecular weight | 100 aa, 11 kDa |
| GenBank accession number | BC113432 |
| Gene symbol | BGLAP |
| Uniprot | P02818 |
| Gene ID (NCBI) | 632 |
| RRID | AB_2879275 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Osteocalcin is a small, highly conserved molecule associated with mineralization of bone matrix. Osteocalcin is specifically expressed in osteoblasts and is the most abundant non-collagenous protein in bone. It regulates the dynamics of new bone formation and bone resorption by interaction with vitamin D, and by influencing the differentiation of osteoblasts. Osteocalcin is also involved in the posttranslational targeting of vitamin K-dependent gamma-carboxylation, which controls blood coagulation.
实验方案
| PRODUCT SPECIFIC PROTOCOLS | |
|---|---|
| IHC protocol for Osteocalcin antibody 23418-1-AP | Download protocol |
| IF protocol for Osteocalcin antibody 23418-1-AP | Download protocol |
| STANDARD PROTOCOLS | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| SPECIES | APPLICATION | TITLE |
|---|---|---|
Colloids Surf B Biointerfaces Bi-layered electrospun nanofibrous membrane with osteogenic and antibacterial properties for guided bone regeneration. | ||
FEBS J Diminished membrane recruitment of Akt is instrumental in alcohol-associated osteopenia via the PTEN/Akt/GSK-3β/β-catenin axis. | ||
Arch Gynecol Obstet Oviductus Ranae protein hydrolyzate prevents menopausal osteoporosis by regulating TGFβ/BMP2 signaling. | ||
Cell Death Differ A novel long noncoding RNA PGC1β-OT1 regulates adipocyte and osteoblast differentiation through antagonizing miR-148a-3p. | ||
J Cell Physiol Reduced PDGF-AA in subchondral bone leads to articular cartilage degeneration after strenuous running. | ||
