Key features and details

• Assay type: Quantitative
• Detection method: Colorimetric/Fluorometric
• Platform: Microplate reader
• Assay time: 40 min
• Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts

• Product name

  Free Glycerol Assay Kit
  See all Glycerol kits

• Detection method

  Colorimetric/Fluorometric

• Sample type

  Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts

• Assay type

  Quantitative

• Sensitivity

  > 1 µM

• Range

  1 µM - 10000 µM

• Assay time

  0h 40m

• Product overview

  Glycerol Assay Kit (ab65337) provides a sensitive, easy assay to measure free glycerol concentration.

  
  

  In the glycerol assay protocol, glycerol is enzymatically oxidized to generate a product which reacts with the probe to generate color (λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm).

  
  

  This glycerol assay kit can detect 50 pmol-10 nmol (or ~1-10000 µM range) of glycerol.

  
  

  Glycerol assay protocol summary:
  - add samples and standards to wells
  - add reaction mix and incubate for 30 min
  - analyze with a microplate reader

• Notes

  This product is manufactured by BioVision, an Abcam company and was previously called K630 Free Glycerol Colorimetric/Fluorometric Assay Kit. K630-100 is the same size as the 100 test size of ab65337.

  Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
  It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

• Platform

  Microplate reader
• Sensitivity: 1 µM