在同一个样本中检测萤火虫和海肾萤光素酶活性

• 海肾萤光素酶内参可提供更准确的数据
• 可直接在生长培养基中直接检测细胞的双报告基因信号
• 每种萤光素酶信号半衰期都可长达2小时
  
  

  An Extended-Life Dual-Luciferase Assay with "Add-Mix-Measure" Convenience

  The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter genes in a single sample. The convenient "add-mix-measure" protocol generates both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed.

  With the Dual-Glo® System, internal controls can be established to minimize sample variability by reducing false-positive and false-negative readings caused by nonspecific factors such as cytotoxicity. In the Dual-Glo® Luciferase Assay, the activity of the primary reporter is correlated with the effect of specific stimuli, and the activity of the co-transfected control reporter provides an internal control to normalize results.

  The system is optimized for batch processing both 96- and 384-well plates and is compatible with a wide variety of mammalian cell culture media. The Dual-Glo® Luciferase Assay System provides high Z´-factors for cell-based, high-throughput screening applications.

  Assay Advantages

  • Increased Precision and Accuracy. Assay normalization using an internal control co-reporter minimizes the effects of variations in cell number and health, transfection efficiency and nonspecific cellular responses.
  • No centrifugation or pre-lysis steps required. Reagents are added directly to growth medium for both reporters.
  • Stable signal allows flexibility for batch or continuous processing of 96- and 384-well plates. Each luminescent signal can be measured for up to 2 hours after reagent addition.
  • Simple, two-step assay is compatible with any luminometer. On-board injectors not required.
  • Wide Dynamic Range allows analysis of high and low reporter activity without sample dilution. Linear over at least 6 logs of enzyme concentration for each reporter.

  Learn more about custom options for this product at: www.promega.com/custom/ 
  Contact Promega for information on bulk purchases.