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anti-PEG的AGP4-PABM-A抗体

anti-PEG的AGP4-PABM-A抗体

AGP4

 

Product No. AGP4-PABM-A

 

Product details

 

AGP4 is a second generation IgM anti-PEG monoclonal antibody. This antibody binds to the PEG backbone and is suitable for capture of PEGylated compounds in ELISA as well as the detection of PEGylated molecules.

A very sensitive ELISA can be achieved utilizing AGP4 as a capture antibody and 3.3-biotin as the detection reagent. Tween-20 can be used in wash buffers for this antibody combination

AGP4 data sheet

Description:

Mouse anti-PEG

Specificity:

Polyethylene glycol

Format:

Purified

Product type:

Monoclonal antibody

Clone:

AGP4

Isotype:

IgM

Species:

Mouse

Price:

$850

Quantity:

500 µg

Example
data sheets:

1. no glycerol
2. with glycerol

AGP4 ELISABinding of AGP4 to immobilized PEG.
Serial dilutions of AGP4 were incubated in ELISA plates coated with 5 µg methoxy-PEG750 or 0.5 µg methoxy-PEG3000, amino-PEG10,000 or methoxy-PEG20,000. Binding of AGP4 to PEG was detected by addition of HRP-labeled anti-rat IgM and ABTS substrate.

 
Sandwich ELISA results for PEGylated proteins and nanoparticles are available in applications

Literature using anti-PEG antibodies is available in publications

Confirmed applications

Use

Yes

No

     Not determined

ELISA

 

 

Western blots

 

 

Flow cytometer

 

 

Immunohistochemistry

 

 

PEG-proteins